Bias is the systematic error of an estimator: whether it tends to over/under estimate the true parameter across repeated samples.
For estimator $\hat\theta$ of a parameter $\theta$, bias is defined as:
\mathbb{E}[\hat{\theta}] - \theta $$
where:
For example, the sample mean $\bar X$ is unbiased for the population mean $\mu$:
$$ \Bbb E[\bar X]=\mu \implies \text{Bias}(\bar X)=0 $$
Bias concerns average behavior over many possible samples, not the error of one particular estimate. An estimator can be biased yet useful if its lower variance producers a lower overall mean squared error:
$$ \text{MSE}(\hat\theta)=\text{Var}(\hat\theta)+\text{Bias}(\theta)^2 $$